Restriction Enzymes
New England Biolabs, Inc. NarI – 2500 units
A restriction endonuclease that recognizes the sequence GG^CG_CC.
New England Biolabs, Inc. HpaII – 10000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. XmaI – 2500 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
New England Biolabs, Inc. NotI – 500 units
A restriction endonuclease that recognizes the sequence GC^GGCC_GC.
New England Biolabs, Inc. NEBuffer™ 1 – 5 ml
New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity. This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation (which is included with NEB restriction enzymes), select NEBuffer 1.1. New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer (NEB #B7204) are available.
New England Biolabs, Inc. BstEII-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GTNACC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. Fpg – 500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. APE 1 – 5000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. APE 1 – 1000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. BspEI – 5000 units
A restriction endonuclease that recognizes the sequence T^CCGG_A.
New England Biolabs, Inc. BslI – 5000 units
A restriction endonuclease that recognizes the sequence CCNN_NNN^NNGG.
New England Biolabs, Inc. I-SceI – 500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. BpmI – 100 units
A restriction endonuclease that recognizes the sequence CTGGAGNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. Acc65I – 10000 units
A restriction endonuclease that recognizes the sequence G^GTAC_C.
New England Biolabs, Inc. EciI – 100 units
A restriction endonuclease that recognizes the sequence GGCGGANNNNNNNNN_NN^.